test menu - AF PCR for IAI Detection
Amniotic Fluid 16S rDNA by Endpoint PCR For detection of Bacterial/Mycoplasma and Ureaplasma species
| Clinical Significance: | Intraamniotic infection is an acute bacterial infection of the amniotic fluid and intrauterine contents that complicates 4 – 10% of all pregnancies. Intraamniotic infection is also an important cause of preterm birth, responsible for 10 – 20% of all preterm births and more than 50% of preterm births occurring before 30 weeks of gestation. Intraamniotic infection often presents as preterm labor. 80% - 90% of cases of intraamniotic infection have no clinical signs or symptoms other than preterm labor. |
| Methodology: | The ProteoGenix amniotic fluid 16S rDNA polymerase chain reaction (PCR) assay provides a qualitative and sensitive tool for detecting the presence of eubacterial DNA, including Mycoplasma and Ureaplasma species in amniotic fluid. The PCR assay amplifies a 500 bp region of the 16S rDNA gene in order to determine the presence or absence of eubacterial DNA. This test employs the endpoint PCR format and uses agarose gel electrophoresis and ethidium bromide dye to visualize PCR products. |
| Test Code: | AFPCR100 |
| Preferred Specimen: | 1.0 mL of Amniotic Fluid |
| Minimum Volume: | 0.5 mL of Amniotic Fluid |
| Transport Container: | ProteoGenix Molecular Transport Tube |
| Transport Temperature: | Room temperature (15°C-30°C) Frozen (-20 °C) |
| Specimen Stability: | Room temperature (15°C-30°C) for 72 hours Frozen (-20 °C) 12 months |
| Limitations: | Non-sterile collection |
| CPT Code: | 87801 (The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.) |
| Price: | $175 |
| Reporting & Turn Time: (PST) | Monday-Friday: specimens received at ProteoGenix Clinical
Laboratory in Costa Mesa, California by 12:00 PM (Pacific Time)
will have results available by 5:00 PM that same day. Specimens received after 12:00 PM, will have results available by 12:00 PM the following day. Specimens received after 12:00 PM on Friday will have results released by 12:00 PM the following Monday. |
| Reference Interval: | Negative |
| Alert Value: | All positive results are considered evidence of Intraamniotic infection and will be communicated to the ordering physician or their designee within one hour of the final report being generated. |
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